Acetylcholinesterase Kit ELISA
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- Antigène Voir toutes Acetylcholinesterase (AChE) Kits ELISA
- Acetylcholinesterase (AChE)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human Acetylcholinesterase/ACHE ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Human Acetylcholinesterase.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product AChE Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Acetylcholinesterase (AChE) Kits ELISA
- Acetylcholinesterase (AChE)
- Autre désignation
- Acetylcholinesterase (ACHE (AChE Produits)
- Synonymes
- ACEE Kit ELISA, ARACHE Kit ELISA, N-ACHE Kit ELISA, YT Kit ELISA, Ache Kit ELISA, GB14873 Kit ELISA, zgc:92550 Kit ELISA, ACE Kit ELISA, Dsim\\GD20515 Kit ELISA, GD20515 Kit ELISA, dsim_GLEANR_4292 Kit ELISA, ache Kit ELISA, mE1a Kit ELISA, mE1b Kit ELISA, mE1c Kit ELISA, mE1c-long Kit ELISA, mE1d Kit ELISA, mE1d' Kit ELISA, mE1e Kit ELISA, arache Kit ELISA, n-ache Kit ELISA, acetylcholinesterase (Cartwright blood group) Kit ELISA, acetylcholinesterase 2 Kit ELISA, acetylcholinesterase Kit ELISA, Acetylcholine esterase Kit ELISA, acetylcholinesterase (Cartwright blood group) L homeolog Kit ELISA, collagen type I alpha 2 chain Kit ELISA, ACHE Kit ELISA, AChE-2 Kit ELISA, Ache Kit ELISA, ache Kit ELISA, Dsim\Ace Kit ELISA, ache.L Kit ELISA, COL1A2 Kit ELISA, ACE-1 Kit ELISA
- UniProt
- P22303
- Pathways
- Skeletal Muscle Fiber Development
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