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ICOSLG Kit ELISA

ICOSLG Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730439
  • Antigène Voir toutes ICOSLG Kits ELISA
    ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
    Reactivité
    • 4
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse B7-H2/ICOS Ligand ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse B7-H2.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes ICOSLG Kits ELISA
    ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
    Autre désignation
    B7-H2 (ICOS Ligand (ICOSLG Produits)
    Synonymes
    ICOSLG Kit ELISA, AU044799 Kit ELISA, B7-H2 Kit ELISA, B7RP-1 Kit ELISA, B7h Kit ELISA, BG071784 Kit ELISA, GI50 Kit ELISA, GL50 Kit ELISA, GL50-B Kit ELISA, ICOS-L Kit ELISA, Icoslg Kit ELISA, LICOS Kit ELISA, Ly115l Kit ELISA, mKIAA0653 Kit ELISA, B7H2 Kit ELISA, B7RP1 Kit ELISA, CD275 Kit ELISA, ICOSL Kit ELISA, Icosl Kit ELISA, RGD1562791 Kit ELISA, inducible T-cell costimulator ligand Kit ELISA, icos ligand Kit ELISA, inducible T-cell co-stimulator ligand Kit ELISA, ICOSLG Kit ELISA, Icosl Kit ELISA, Icoslg Kit ELISA
    UniProt
    Q9JHJ8
    Pathways
    Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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