GAPDH Kit ELISA

N° du produit ABIN6730487

Détail du produit GAPDH Kit ELISA

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse GAPDH ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse GAPDH.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH (GAPDH Produits)

Synonymes: G3PD Kit ELISA, GAPD Kit ELISA, Gapd Kit ELISA, BEST:GH12586 Kit ELISA, CG12055 Kit ELISA, Dmel\\CG12055 Kit ELISA, GA3PDH Kit ELISA, GADPH Kit ELISA, GAP Kit ELISA, GAPDH Kit ELISA, GAPDH I Kit ELISA, GAPDH-1 Kit ELISA, GAPDH1 Kit ELISA, GAPDHI Kit ELISA, Gapdh Kit ELISA, Gapdh-1 Kit ELISA, Gapdh43E Kit ELISA, gadph Kit ELISA, gapdh Kit ELISA, gapdh-1 Kit ELISA, gh12586 Kit ELISA, cb609 Kit ELISA, gapd Kit ELISA, mg:bb02e05 Kit ELISA, wu:fb33a10 Kit ELISA, wu:ft80f05 Kit ELISA, KNC-NDS6 Kit ELISA, g3pd Kit ELISA, G3PDH Kit ELISA, glyceraldehyde-3-phosphate dehydrogenase Kit ELISA, Glyceraldehyde 3 phosphate dehydrogenase 1 Kit ELISA, glyceraldehyde-3-phosphate dehydrogenase S homeolog Kit ELISA, glyceraldehyde-3-phosphate dehydrogenase, type I Kit ELISA, olfactory receptor 8K3 Kit ELISA, GAPDH Kit ELISA, Gapdh Kit ELISA, Gapdh1 Kit ELISA, gapdh Kit ELISA, gapdh.S Kit ELISA, gapDH Kit ELISA, LOC100404960 Kit ELISA, LOC614985 Kit ELISA

UniProt: P16858