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LEFTY1 Kit ELISA

LEFTY1 Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730499
  • Antigène Voir toutes LEFTY1 Kits ELISA
    LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))
    Reactivité
    • 2
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse Lefty-1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse Lefty-1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product LEFTY1 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes LEFTY1 Kits ELISA
    LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))
    Autre désignation
    Lefty-1 (LEFTY1 Produits)
    Synonymes
    atv Kit ELISA, cb73 Kit ELISA, antivin Kit ELISA, ik:tdsubc_2b12 Kit ELISA, xx:tdsubc_2b12 Kit ELISA, LEFTY1 Kit ELISA, AI450052 Kit ELISA, Ebaf Kit ELISA, Leftb Kit ELISA, Stra3 Kit ELISA, Tgfb4 Kit ELISA, lefty Kit ELISA, lefty-1 Kit ELISA, RGD1561867 Kit ELISA, LEFTB Kit ELISA, LEFTYB Kit ELISA, lefty1 Kit ELISA, signaling molecule lefty1 Kit ELISA, left-right determination factor 1 Kit ELISA, left right determination factor 1 Kit ELISA, lft1 Kit ELISA, lefty1 Kit ELISA, LOC699924 Kit ELISA, LEFTY1 Kit ELISA, LOC100408003 Kit ELISA, LOC100597378 Kit ELISA, Lefty1 Kit ELISA
    UniProt
    Q64280
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