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UBL5 Kit ELISA

UBL5 Reactivité: Humain, Souris, Rat Colorimetric Competition ELISA 0.1 ng/mL - 1000 ng/mL Cell Culture Supernatant, Serum
N° du produit ABIN6730523
  • Antigène Voir toutes UBL5 Kits ELISA
    UBL5 (Ubiquitin-Like 5 (UBL5))
    Reactivité
    Humain, Souris, Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    0.1 ng/mL - 1000 ng/mL
    Seuil minimal de détection
    0.1 ng/mL
    Application
    ELISA
    Fonction
    Human/Mouse/Rat Ubiquitin-like protein 5 EIA Kit optimized for serum and cell culture media. Competition-based ELISA on a 96-well strip plate.
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This EIA kit is designed to detect human, mouse, and rat Ubiquitin-like protein 5
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    Préparation des réactifs

    Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    Date de péremption
    6 months
  • Antigène Voir toutes UBL5 Kits ELISA
    UBL5 (Ubiquitin-Like 5 (UBL5))
    Autre désignation
    Ubiquitin-like protein 5 (UBL5 Produits)
    Synonymes
    HUB1 Kit ELISA, 1110030M22Rik Kit ELISA, beacon Kit ELISA, UBL5 Kit ELISA, DKFZp469G145 Kit ELISA, K5J14.10 Kit ELISA, K5J14_10 Kit ELISA, ubiquitin-like protein 5 Kit ELISA, ubl5 Kit ELISA, ubiquitin like 5 Kit ELISA, ubiquitin-like 5 Kit ELISA, Ubiquitin-like protein 5 Kit ELISA, ubiquitin-like protein 5 Kit ELISA, zgc:66388 Kit ELISA, ubiquitin like 5 S homeolog Kit ELISA, UBL5 Kit ELISA, Ubl5 Kit ELISA, ubl-5 Kit ELISA, zgc:66388 Kit ELISA, ubl5.S Kit ELISA
    ID gène
    59286
    UniProt
    Q9BZL1
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