TPT1 Kit ELISA

N° du produit ABIN6730534

Détail du produit TPT1 Kit ELISA

Antigène: TPT1 (Tumor Protein, Translationally-Controlled 1 (TPT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human TPT1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human TPT1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TPT1 (Tumor Protein, Translationally-Controlled 1 (TPT1))

Autre désignation: TPT1 (TPT1 Produits)

Synonymes: HRF Kit ELISA, TCTP Kit ELISA, p02 Kit ELISA, Trt Kit ELISA, p21 Kit ELISA, p23 Kit ELISA, TPT1 Kit ELISA, TCT1 Kit ELISA, tctp Kit ELISA, fa28a02 Kit ELISA, id:ibd2078 Kit ELISA, wu:fa28a02 Kit ELISA, PCHK23 Kit ELISA, hrf Kit ELISA, MGC79720 Kit ELISA, Tpt1 Kit ELISA, translationally controlled tumor protein Kit ELISA, CG4800 Kit ELISA, Dmel\\CG4800 Kit ELISA, dTCTP Kit ELISA, Tctp Kit ELISA, tumor protein, translationally-controlled 1 Kit ELISA, tumor protein, translationally-controlled 1 L homeolog Kit ELISA, translationally-controlled tumor protein Kit ELISA, translationally controlled tumor protein Kit ELISA, translationally controlled tumor-like protein Kit ELISA, Translationally controlled tumor protein Kit ELISA, translationally-controlled tumor protein homolog Kit ELISA, P23 tumor protein-like Kit ELISA, TPT1 Kit ELISA, Tpt1 Kit ELISA, tpt1 Kit ELISA, tpt1.L Kit ELISA, LOC711798 Kit ELISA, TCTP Kit ELISA, LOC548038 Kit ELISA, Tctp Kit ELISA, LOC108708523 Kit ELISA, LOC109404863 Kit ELISA, LOC105389397 Kit ELISA, LOC102577554 Kit ELISA

UniProt: P13693

Pathways: Response to Water Deprivation, Regulation of Cell Size, Negative Regulation of intrinsic apoptotic Signaling