ARL2BP Kit ELISA

N° du produit ABIN6730576

Détail du produit ARL2BP Kit ELISA

Antigène: ARL2BP (ADP-Ribosylation Factor-Like 2 Binding Protein (ARL2BP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ARL2BP ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human ARL2BP.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: ARL2BP (ADP-Ribosylation Factor-Like 2 Binding Protein (ARL2BP))

Autre désignation: ARL2BP (ARL2BP Produits)

Synonymes: arl2bp Kit ELISA, MGC81575 Kit ELISA, MGC84796 Kit ELISA, MGC130948 Kit ELISA, MGC107872 Kit ELISA, ARL2BP Kit ELISA, BART Kit ELISA, BART1 Kit ELISA, 1700010P10Rik Kit ELISA, 1700027H16Rik Kit ELISA, 6330544B05Rik Kit ELISA, AI482273 Kit ELISA, AI849834 Kit ELISA, Bart1 Kit ELISA, zgc:66308 Kit ELISA, zgc:77496 Kit ELISA, ADP ribosylation factor like GTPase 2 binding protein L homeolog Kit ELISA, ADP ribosylation factor like GTPase 2 binding protein Kit ELISA, ADP-ribosylation factor-like 2 binding protein Kit ELISA, ADP-ribosylation factor like GTPase 2 binding protein Kit ELISA, arl2bp.L Kit ELISA, arl2bp Kit ELISA, ARL2BP Kit ELISA, Arl2bp Kit ELISA

UniProt: Q9Y2Y0

Pathways: Maintenance of Protein Location