GOT1 Kit ELISA

N° du produit ABIN6730579

Détail du produit GOT1 Kit ELISA

Antigène: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human GOT1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human GOT1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Autre désignation: GOT1 (GOT1 Produits)

Synonymes: ASTQTL1 Kit ELISA, GIG18 Kit ELISA, cAspAT Kit ELISA, cCAT Kit ELISA, AST Kit ELISA, ISSD Kit ELISA, NSD Kit ELISA, SD Kit ELISA, SIALIN Kit ELISA, SIASD Kit ELISA, SLD Kit ELISA, xr406 Kit ELISA, CASPAT Kit ELISA, ASPARTATE AMINOTRANSFERASE Kit ELISA, ATAAT Kit ELISA, MATERNAL EFFECT EMBRYO ARREST 17 Kit ELISA, MEE17 Kit ELISA, T26C19.9 Kit ELISA, T26C19_9 Kit ELISA, aspartate aminotransferase Kit ELISA, aatA Kit ELISA, 83.t00021 Kit ELISA, AI789014 Kit ELISA, Got-1 Kit ELISA, Aspat Kit ELISA, Gaspat Kit ELISA, AL022787 Kit ELISA, FABP-pm Kit ELISA, Got-2 Kit ELISA, mAspAT Kit ELISA, ASPATA Kit ELISA, glutamic-oxaloacetic transaminase 1 Kit ELISA, solute carrier family 17 member 5 Kit ELISA, glutamic-oxaloacetic transaminase 1 homeolog Kit ELISA, aspartate aminotransferase Kit ELISA, glutamic-oxaloacetic transaminase 1, soluble Kit ELISA, glutamic-oxaloacetic transaminase 2 Kit ELISA, glutamatic-oxaloacetic transaminase 2, mitochondrial Kit ELISA, GOT1 Kit ELISA, SLC17A5 Kit ELISA, got1.S Kit ELISA, AAT Kit ELISA, aspC Kit ELISA, EHI_006080 Kit ELISA, AST2 Kit ELISA, ast2 Kit ELISA, got1 Kit ELISA, Got1 Kit ELISA, GOT2 Kit ELISA, Got2 Kit ELISA

UniProt: P17174

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process