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FABP4 Kit ELISA

FABP4 Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730639
  • Antigène Voir toutes FABP4 Kits ELISA
    FABP4 (Fatty Acid Binding Protein 4, Adipocyte (FABP4))
    Reactivité
    • 8
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse FABP4/A-FABP ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse FABP4.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product FABP4 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes FABP4 Kits ELISA
    FABP4 (Fatty Acid Binding Protein 4, Adipocyte (FABP4))
    Autre désignation
    FABP4 (A-FABP (FABP4 Produits)
    Synonymes
    A-FABP Kit ELISA, AFABP Kit ELISA, ALBP Kit ELISA, aP2 Kit ELISA, LOC100223994 Kit ELISA, FABP Kit ELISA, AP2 Kit ELISA, FABP3 Kit ELISA, FABP4 Kit ELISA, MGC84940 Kit ELISA, fabp4 Kit ELISA, 422/aP2 Kit ELISA, ALBP/Ap2 Kit ELISA, Ap2 Kit ELISA, Lbpl Kit ELISA, Albp Kit ELISA, fatty acid binding protein 4 Kit ELISA, fatty acid-binding protein, adipocyte Kit ELISA, fatty acid binding protein 4, adipocyte Kit ELISA, fatty acid binding protein 4, adipocyte L homeolog Kit ELISA, Fatty acid-binding protein, adipocyte Kit ELISA, adipocyte fatty acid-binding protein Kit ELISA, adipocyte fatty acid binding protein Kit ELISA, FABP4 Kit ELISA, LOC100223994 Kit ELISA, fabp4.L Kit ELISA, fabp4 Kit ELISA, Fabp4 Kit ELISA, LOC100732353 Kit ELISA, LOC100861279 Kit ELISA, LOC100057425 Kit ELISA, LOC395224 Kit ELISA
    UniProt
    P04117
    Pathways
    Brown Fat Cell Differentiation
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