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IL36A/IL1F6 Kit ELISA
IL1F6
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730743
Détail du produit IL36A/IL1F6 Kit ELISA
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Antigène
Voir toutes IL36A/IL1F6 (IL1F6) Kits ELISA
IL36A/IL1F6 (IL1F6)
(Interleukin 1 Family, Member 6 (IL1F6))
Reactivité
Toutes les réactivités sur IL36A/IL1F6 Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse IL-36 alpha/IL-1F6 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse IL-36 alpha.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes IL36A/IL1F6 (IL1F6) Kits ELISA
IL36A/IL1F6 (IL1F6)
(Interleukin 1 Family, Member 6 (IL1F6))
Autre désignation
IL-36 alpha (IL-1F6 (IL1F6 Produits )
Synonymes
Il1f6 Kit ELISA, Fil1 Kit ELISA, IL-1H1 Kit ELISA, IL1RP2 Kit ELISA, Il1f9 Kit ELISA, Il36a Kit ELISA, FIL1 Kit ELISA, FIL1(EPSILON) Kit ELISA, FIL1E Kit ELISA, IL-1F6 Kit ELISA, IL1(EPSILON) Kit ELISA, IL1F6 Kit ELISA, interleukin 36, alpha Kit ELISA, interleukin 1 family, member 6 (epsilon) Kit ELISA, interleukin 1 family, member 6 Kit ELISA, interleukin 36 alpha Kit ELISA, Il36a Kit ELISA, IL1F6 Kit ELISA, IL36A Kit ELISA, Il1f6 Kit ELISA
UniProt
Q9JLA2
Pathways
Cancer Immune Checkpoints
Vus récemment
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