NPM1 Kit ELISA

N° du produit ABIN6730755

Détail du produit NPM1 Kit ELISA

Antigène: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human NPM1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human NPM1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Autre désignation: NPM1 (NPM1 Produits)

Synonymes: B23 Kit ELISA, NPM Kit ELISA, NO38 Kit ELISA, Npm Kit ELISA, B23NP Kit ELISA, npm4 Kit ELISA, npm Kit ELISA, CG7917 Kit ELISA, CRP1 Kit ELISA, Crp1 Kit ELISA, Dmel\\CG7917 Kit ELISA, dNLP Kit ELISA, dNLP-S Kit ELISA, p22 Kit ELISA, npm1 Kit ELISA, sb:cb487 Kit ELISA, nucleophosmin 1 Kit ELISA, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog Kit ELISA, nucleophosmin 1b Kit ELISA, Nucleophosmin Kit ELISA, nucleophosmin Kit ELISA, nucleophosmin (nucleolar phosphoprotein B23, numatrin) Kit ELISA, Nucleoplasmin Kit ELISA, nucleophosmin 1a Kit ELISA, NPM1 Kit ELISA, Npm1 Kit ELISA, npm1.L Kit ELISA, npm1b Kit ELISA, npm Kit ELISA, Nlp Kit ELISA, npm1a Kit ELISA

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly