PLA2G2A Kit ELISA

N° du produit ABIN6730778

Le kit ELISA PLA2G2A (ABIN6730778) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris PLA2G2A.

Aperçu rapide pour PLA2G2A Kit ELISA (ABIN6730778)

Antigène: PLA2G2A (Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 2.56 pg/mL - 1500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit PLA2G2A Kit ELISA

Seuil minimal de détection: 2.56 pg/mL

Fonction: The Mouse PLA2G2A ELISA Kit is a pre-coated sandwich-based assay kit designed to quantitatively measure PLA2G2A levels in mouse cell culture supernatants, plasma, and serum.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects mouse PLA2G2A. Other species not determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)
• Adhesive Sealing Film

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard or sample dilutions
• Plastic wrap

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: PLA2G2A (Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A))

Autre désignation: PLA2G2A

Sujet: Gene Symbols: Phospholipase A2, membrane associated (EC 3.1.1.4) (Enhancing factor) (EF) (GIIC sPLA2) (Group IIA phospholipase A2) (Phosphatidylcholine 2-acylhydrolase 2A)

UniProt: P31482

Pathways: Stem Cell Maintenance, Inositol Metabolic Process