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TSC22D1 Kit ELISA

TSC22D1 Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730783
  • Antigène Voir toutes TSC22D1 Kits ELISA
    TSC22D1 (TSC22 Domain Family, Member 1 (TSC22D1))
    Reactivité
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    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse TSC22D1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse TSC22D1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product TSC22D1 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes TSC22D1 Kits ELISA
    TSC22D1 (TSC22 Domain Family, Member 1 (TSC22D1))
    Autre désignation
    TSC22D1 (TSC22D1 Produits)
    Synonymes
    Ptg-2 Kit ELISA, TGFB1I4 Kit ELISA, TSC22 Kit ELISA, AA589566 Kit ELISA, AW105905 Kit ELISA, Egr5 Kit ELISA, TSC-22 Kit ELISA, Tgfb1i4 Kit ELISA, Tsc Kit ELISA, Tsc22 Kit ELISA, tsc22 Kit ELISA, TS22A Kit ELISA, fj34d05 Kit ELISA, wu:fj34d05 Kit ELISA, zgc:65869 Kit ELISA, zgc:85857 Kit ELISA, TSC22D1 Kit ELISA, tsc22d1 Kit ELISA, MGC69210 Kit ELISA, MGC186361 Kit ELISA, DKFZp459J163 Kit ELISA, TSC22 domain family member 1 Kit ELISA, TSC22 domain family, member 1 Kit ELISA, TSC22 domain family member 1 L homeolog Kit ELISA, TSC22D1 Kit ELISA, Tsc22d1 Kit ELISA, tsc22d1.L Kit ELISA, tsc22d1 Kit ELISA
    UniProt
    P62500
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