ACTH Kit ELISA

N° du produit ABIN6730843

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris, Humain et Rat ACTH.

Aperçu rapide pour ACTH Kit ELISA (ABIN6730843)

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivité: Souris, Humain, Rat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1 ng/mL - 1000 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit ACTH Kit ELISA

Seuil minimal de détection: 0.1 ng/mL

Fonction: Human/Mouse/Rat Adrenocorticotropic Hormone EIA Kit optimized for serum and cell culture medium. Competition-based ELISA

Analytical Method: Quantitative

Specificité: This EIA kit is designed to detect human, mouse, and rat ACTH

Sensibilité: 0.5 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Suggested Plasma Dilution: Human: 2X / Mouse: 2X / Rat: 2X

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

• Prepare all reagents, samples and standards as instructed.
• Add 100 μL detection antibody to each well.
• Incubate 1.5 h at RT or O/N at 4 °C.
• Add 100 μL standard or sample to each well.
• Incubate 2.5 h at RT.
• Add 100 μL prepared streptavidin solution.
• Incubate 45 min at RT.
• Add 100 μL TMB One-Step Substrate Reagent to each well.
• Incubate 30 min at RT.
• Add 50 μL Stop Solution to each well.
• Read plate at 450 nm immediately.

Préparation des réactifs:

Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, Biotinylated ACTH peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Autre désignation: Adrenocorticotropic hormone

Classe de substances: Hormone

Sujet:

Gene Symbols: POMC

Background: Adrenocorticotropic hormone (ACTH, also adrenocorticotropin, corticotropin) is a 39 amino acids polypeptide tropic hormone. ACTH is synthesized from pre-pro-opiomelanocortin (pre-POMC), which is proteolytically cleaved by endopeptidases to yield various polypeptide fragments with varying physiological activity. ACTH is secreted by the anterior pituitary gland. It stimulates secretion of glucocorticoid steroid hormones from adrenal cortex cells, especially in the zona fasciculata of the adrenal glands. It influences steroid hormone secretion by both rapid short-term mechanisms and long-term actions. ACTH is also used as a medication and diagnostic agent.

Protein Name (Synonyms): Pro-opiomelanocortin (POMC) (Corticotropin-lipotropin) [Cleaved into: NPP, Melanotropin gamma (Gamma-MSH), Potential peptide, Corticotropin (Adrenocorticotropic hormone) (ACTH), Melanotropin alpha (Alpha-MSH), Corticotropin-like intermediary peptide (CLIP), Lipotropin beta (Beta-LPH), Lipotropin gamma (Gamma-LPH), Melanotropin beta (Beta-MSH), Beta-endorphin, Met-enkephalin]

ID gène: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity