Hemoglobin Subunit beta Kit ELISA

N° du produit ABIN6810489

Détail du produit Hemoglobin Subunit beta Kit ELISA

Antigène: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human HbA1c/HBB ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human HbA1c.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Autre désignation: HBB (HBB Produits)

Synonymes: HBB Kit ELISA, HBB1 Kit ELISA, HBBA Kit ELISA, HBBB Kit ELISA, CD113t-C Kit ELISA, beta-globin Kit ELISA, hbb Kit ELISA, hemoglobin, beta Kit ELISA, hemoglobin subunit beta Kit ELISA, hemoglobin subunit beta-like Kit ELISA, beta globin Kit ELISA, hemoglobin beta chain complex Kit ELISA, hemoglobin subunit beta-1 Kit ELISA, HBB Kit ELISA, HBB2 Kit ELISA, LOC100720944 Kit ELISA, LOC480784 Kit ELISA, Hbb Kit ELISA, LOC100136576 Kit ELISA, LOC101098159 Kit ELISA, LOC100976465 Kit ELISA, LOC100410611 Kit ELISA, LOC105468624 Kit ELISA

Classe de substances: Viral Protein

UniProt: P68871