SAE1 Kit ELISA
SAE1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951062
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Antigène
Voir toutes SAE1 Kits ELISA
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SAE1
(SUMO1 Activating Enzyme Subunit 1 (SAE1))
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Reactivité
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Humain
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Human SAE1 ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Indications d'application
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Optimal working dilution should be determined by the investigator.
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Date de péremption
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6 months
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Antigène
Voir toutes SAE1 Kits ELISA
-
SAE1
(SUMO1 Activating Enzyme Subunit 1 (SAE1))
-
Autre désignation
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SAE1 (SAE1 Produits)
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Synonymes
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AOS1 Kit ELISA, HSPC140 Kit ELISA, SUA1 Kit ELISA, UBLE1A Kit ELISA, 2400010M20Rik Kit ELISA, 2610044L12Rik Kit ELISA, AL033372 Kit ELISA, AW743391 Kit ELISA, D7Ertd177e Kit ELISA, Sua1 Kit ELISA, Uble1a Kit ELISA, Aos1p Kit ELISA, Sua1p Kit ELISA, aos Kit ELISA, sae2a Kit ELISA, uble1a Kit ELISA, wu:fa28b04 Kit ELISA, zgc:86633 Kit ELISA, SUMO1 activating enzyme subunit 1 Kit ELISA, SUMO1 activating enzyme subunit 1 L homeolog Kit ELISA, SAE1 Kit ELISA, Sae1 Kit ELISA, sae1.L Kit ELISA, sae1 Kit ELISA
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ID gène
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10055
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UniProt
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Q9UBE0
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