DARS Kit ELISA

N° du produit ABIN6951073

Détail du produit DARS Kit ELISA

Antigène: DARS (Aspartyl-tRNA Synthetase (DARS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human DARS ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: DARS (Aspartyl-tRNA Synthetase (DARS))

Autre désignation: DARS (DARS Produits)

Synonymes: dars Kit ELISA, MGC53970 Kit ELISA, MGC76305 Kit ELISA, DARS Kit ELISA, Aats-Asp Kit ELISA, AspRS Kit ELISA, CG3821 Kit ELISA, DRS Kit ELISA, Dmel\\CG3821 Kit ELISA, aats-asp Kit ELISA, anon-EST:Liang-2.49 Kit ELISA, cDNA1 Kit ELISA, clone 2.49 Kit ELISA, l(2)49Db Kit ELISA, l(2)k04508 Kit ELISA, l(2)v27 Kit ELISA, l(2)vr1 Kit ELISA, poney Kit ELISA, vr1 Kit ELISA, MGC80207 Kit ELISA, wu:fc17a11 Kit ELISA, zgc:154056 Kit ELISA, ECK1867 Kit ELISA, JW1855 Kit ELISA, tls Kit ELISA, HBSL Kit ELISA, 5730439G15Rik Kit ELISA, DRS1 Kit ELISA, aspartyl-tRNA synthetase S homeolog Kit ELISA, aspartyl-tRNA synthetase Kit ELISA, Aspartyl-tRNA synthetase Kit ELISA, aspartyl-tRNA synthetase L homeolog Kit ELISA, aspartate--tRNA(Asn) ligase Kit ELISA, Aspartate--tRNA ligase, cytoplasmic Kit ELISA, dars.S Kit ELISA, dars Kit ELISA, DARS Kit ELISA, AspRS Kit ELISA, dars.L Kit ELISA, aspS Kit ELISA, RMDY18_09660 Kit ELISA, Tbis_2053 Kit ELISA, Slip_0775 Kit ELISA, Aats-asp Kit ELISA, MFER_RS02995 Kit ELISA, CKC_01935 Kit ELISA, Dars Kit ELISA, dars-1 Kit ELISA

ID gène: 1615

UniProt: P14868