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COPZ1 Kit ELISA

COPZ1 Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951108
  • Antigène Tous les produits COPZ1
    COPZ1 (Coatomer Protein Complex, Subunit zeta 1 (COPZ1))
    Reactivité
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Rat COPZ1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Tous les produits COPZ1
    COPZ1 (Coatomer Protein Complex, Subunit zeta 1 (COPZ1))
    Autre désignation
    COPZ1 (COPZ1 Produits)
    Synonymes
    COPZ Kit ELISA, T7P1.11 Kit ELISA, T7P1_11 Kit ELISA, HSPC181 Kit ELISA, zeta-COP Kit ELISA, zeta1-COP Kit ELISA, 5930435A22Rik Kit ELISA, AA407760 Kit ELISA, D4Ertd360e Kit ELISA, coatomer protein complex, subunit zeta 1 Kit ELISA, coatomer protein complex subunit zeta 1 Kit ELISA, Coatomer protein complex, subunit zeta 1 Kit ELISA, SNARE-like superfamily protein Kit ELISA, coatomer subunit zeta-1 Kit ELISA, Copz1 Kit ELISA, COPZ1 Kit ELISA, CopZ Kit ELISA, copz1 Kit ELISA, AT1G60970 Kit ELISA, CpipJ_CPIJ003005 Kit ELISA, Tsp_01470 Kit ELISA
    ID gène
    315345
    UniProt
    D4A8T3
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