Beclin 1 Kit ELISA
-
- Antigène Voir toutes Beclin 1 (BECN1) Kits ELISA
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human Beclin ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product BECN1 Kit ELISA
-
-
- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Plaque
- Pre-coated
- Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Date de péremption
- 6 months
-
- Antigène Voir toutes Beclin 1 (BECN1) Kits ELISA
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
- Autre désignation
- Beclin (BECN1 Produits)
- Synonymes
- beclin-1 Kit ELISA, atg6 Kit ELISA, vps30 Kit ELISA, beclin1 Kit ELISA, Beclin-1 Kit ELISA, becn1 Kit ELISA, zgc:73097 Kit ELISA, ATG6 Kit ELISA, VPS30 Kit ELISA, 4921513J16Rik Kit ELISA, 5430417M23Rik Kit ELISA, Atg6 Kit ELISA, beclin 1 Kit ELISA, beclin 1, autophagy related Kit ELISA, beclin 1 protein Kit ELISA, beclin-1 Kit ELISA, beclin family protein Kit ELISA, beclin 1, autophagy related L homeolog Kit ELISA, BECN1 Kit ELISA, becn1 Kit ELISA, atg6 Kit ELISA, CpipJ_CPIJ011087 Kit ELISA, PTRG_07825 Kit ELISA, SJAG_01876 Kit ELISA, Becn1 Kit ELISA, becn1.L Kit ELISA
- ID gène
- 8678
- UniProt
- Q14457
- Pathways
- Autophagy
-