STXBP1 Kit ELISA

N° du produit ABIN6951178

Détail du produit STXBP1 Kit ELISA

Antigène: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human STXBP1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Autre désignation: STXBP1 (STXBP1 Produits)

Synonymes: MUNC18-1 Kit ELISA, NSEC1 Kit ELISA, P67 Kit ELISA, RBSEC1 Kit ELISA, UNC18 Kit ELISA, AI317162 Kit ELISA, AI326233 Kit ELISA, MMS10-G Kit ELISA, Ms10g Kit ELISA, Munc-18a Kit ELISA, Munc18-1 Kit ELISA, N-sec1 Kit ELISA, Rb-sec1 Kit ELISA, Sxtbp1 Kit ELISA, Unc18-1 Kit ELISA, Unc18h Kit ELISA, nsec1 Kit ELISA, fj43h10 Kit ELISA, stxbp1 Kit ELISA, wu:fj36d12 Kit ELISA, wu:fj43h10 Kit ELISA, zgc:114171 Kit ELISA, rop Kit ELISA, unc18 Kit ELISA, hunc18 Kit ELISA, rbsec1 Kit ELISA, munc18-1 Kit ELISA, MGC146482 Kit ELISA, ANC18HA Kit ELISA, NSEC1A Kit ELISA, Sec1 Kit ELISA, n-sec1 Kit ELISA, nSec1 Kit ELISA, rbSec1 Kit ELISA, rbSec1A Kit ELISA, rbSec1B Kit ELISA, UNC18A Kit ELISA, si:rp71-10d23.3 Kit ELISA, syntaxin binding protein 1 Kit ELISA, syntaxin binding protein 1a Kit ELISA, syntaxin binding protein 1 L homeolog Kit ELISA, syntaxin binding protein 1b Kit ELISA, STXBP1 Kit ELISA, Stxbp1 Kit ELISA, stxbp1a Kit ELISA, stxbp1 Kit ELISA, Tb09.160.0780 Kit ELISA, stxbp1.L Kit ELISA, stxbp1b Kit ELISA

ID gène: 6812

UniProt: P61764

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport