OPTN Kit ELISA
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- Antigène Voir toutes OPTN Kits ELISA
- OPTN (Optineurin (OPTN))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human OPTN ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product OPTN Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Date de péremption
- 6 months
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- Antigène Voir toutes OPTN Kits ELISA
- OPTN (Optineurin (OPTN))
- Autre désignation
- OPTN (OPTN Produits)
- Synonymes
- nrp Kit ELISA, fip2 Kit ELISA, hip7 Kit ELISA, hypl Kit ELISA, glc1e Kit ELISA, tfiiia-intp Kit ELISA, ALS12 Kit ELISA, FIP2 Kit ELISA, GLC1E Kit ELISA, HIP7 Kit ELISA, HYPL Kit ELISA, NRP Kit ELISA, TFIIIA-INTP Kit ELISA, Fip2 Kit ELISA, 4930441O07Rik Kit ELISA, fj52f04 Kit ELISA, si:ch211-240l19.3 Kit ELISA, wu:fj52f04 Kit ELISA, zgc:66386 Kit ELISA, zgc:77868 Kit ELISA, optineurin Kit ELISA, optineurin L homeolog Kit ELISA, OPTN Kit ELISA, optn Kit ELISA, Optn Kit ELISA, optn.L Kit ELISA
- ID gène
- 10133
- UniProt
- Q96CV9
- Pathways
- M Phase
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