PMVK Kit ELISA

N° du produit ABIN6951213

Détail du produit PMVK Kit ELISA

Antigène: PMVK (Phosphomevalonate Kinase (PMVK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human PMVK ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: PMVK (Phosphomevalonate Kinase (PMVK))

Autre désignation: PMVK (PMVK Produits)

Synonymes: MPK_Bm Kit ELISA, F5M6.9 Kit ELISA, F5M6_9 Kit ELISA, zgc:162701 Kit ELISA, HUMPMKI Kit ELISA, PMK Kit ELISA, PMKA Kit ELISA, PMKASE Kit ELISA, 1110011E12Rik Kit ELISA, 2900002L22Rik Kit ELISA, AV001327 Kit ELISA, phosphomevalonate kinase Kit ELISA, GHMP kinase family protein Kit ELISA, PMVK Kit ELISA, Mpk Kit ELISA, AT1G31910 Kit ELISA, CpipJ_CPIJ015413 Kit ELISA, Bm1_05275 Kit ELISA, pmvk Kit ELISA, LOAG_11811 Kit ELISA, Tsp_06629 Kit ELISA, LOC100282968 Kit ELISA, Pmvk Kit ELISA

ID gène: 10654

UniProt: Q15126