TFF1 Kit ELISA
-
- Antigène Voir toutes TFF1 Kits ELISA
- TFF1 (Trefoil Factor 1 (TFF1))
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human TFF1 ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product TFF1 Kit ELISA
-
-
- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Plaque
- Pre-coated
- Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Date de péremption
- 6 months
-
- Antigène Voir toutes TFF1 Kits ELISA
- TFF1 (Trefoil Factor 1 (TFF1))
- Autre désignation
- TFF1 (TFF1 Produits)
- Synonymes
- BCEI Kit ELISA, D21S21 Kit ELISA, HP1.A Kit ELISA, HPS2 Kit ELISA, pNR-2 Kit ELISA, pS2 Kit ELISA, Bcei Kit ELISA, PS2 Kit ELISA, bcei Kit ELISA, d21s21 Kit ELISA, hp1.a Kit ELISA, hps2 Kit ELISA, p1 Kit ELISA, p1-a Kit ELISA, pnr-2 Kit ELISA, ps2 Kit ELISA, tff1 Kit ELISA, xP1 Kit ELISA, xp1-L Kit ELISA, xp1 Kit ELISA, TFF1 Kit ELISA, Ps2 Kit ELISA, trefoil factor 1 Kit ELISA, trefoil factor 1 S homeolog Kit ELISA, trefoil factor 1, gene 2 S homeolog Kit ELISA, TFF1 Kit ELISA, Tff1 Kit ELISA, tff1.S Kit ELISA, tff1.2.S Kit ELISA, LOC100358238 Kit ELISA
- ID gène
- 7031
- UniProt
- P04155
- Pathways
- EGFR Signaling Pathway
-