Ribokinase Kit ELISA

N° du produit ABIN6951225

Détail du produit Ribokinase Kit ELISA

Antigène: Ribokinase (RBKS)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human RBKS ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: Ribokinase (RBKS)

Autre désignation: RBKS (RBKS Produits)

Synonymes: RBSK Kit ELISA, 5230400M11Rik Kit ELISA, AI326806 Kit ELISA, zgc:86813 Kit ELISA, MGC131284 Kit ELISA, F20D23.14 Kit ELISA, F20D23_14 Kit ELISA, 20.t00015 Kit ELISA, ribokinase Kit ELISA, Ribokinase Kit ELISA, ribokinase L homeolog Kit ELISA, pfkB-like carbohydrate kinase family protein Kit ELISA, RBKS Kit ELISA, Rbks Kit ELISA, F07A11.5 Kit ELISA, rbks Kit ELISA, rbks.L Kit ELISA, AT1G17160 Kit ELISA, EHI_138020 Kit ELISA, Tb11.03.0090 Kit ELISA, Mrub_0200 Kit ELISA, rbkS Kit ELISA, Ndas_4158 Kit ELISA, Mesil_0220 Kit ELISA, Arch_1729 Kit ELISA, Trad_0706 Kit ELISA, Halhy_5191 Kit ELISA, Mesop_4859 Kit ELISA, Theth_0467 Kit ELISA

ID gène: 64080

UniProt: Q9H477