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SRI Kit ELISA

SRI Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951262
  • Antigène Voir toutes SRI Kits ELISA
    SRI (Sorcin (SRI))
    Reactivité
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human SRI ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product SRI Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes SRI Kits ELISA
    SRI (Sorcin (SRI))
    Autre désignation
    SRI (SRI Produits)
    Synonymes
    zgc:64070 Kit ELISA, sri Kit ELISA, SRI Kit ELISA, CP-22 Kit ELISA, CP22 Kit ELISA, SCN Kit ELISA, V19 Kit ELISA, 2210417O06Rik Kit ELISA, 2900070H08Rik Kit ELISA, Sor Kit ELISA, sorcin Kit ELISA, Sorcin Kit ELISA, sorcin L homeolog Kit ELISA, sorcin S homeolog Kit ELISA, SRI Kit ELISA, sri Kit ELISA, EDI_060410 Kit ELISA, EDI_160670 Kit ELISA, EDI_002860 Kit ELISA, EDI_076560 Kit ELISA, CpipJ_CPIJ004933 Kit ELISA, CpipJ_CPIJ008581 Kit ELISA, Tsp_05707 Kit ELISA, sri.L Kit ELISA, sri.S Kit ELISA, Sri Kit ELISA
    ID gène
    6717
    UniProt
    P30626
    Pathways
    Transition Metal Ion Homeostasis, Maintenance of Protein Location, Negative Regulation of Transporter Activity
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