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NCF2 Kit ELISA

NCF2 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951326
  • Antigène Voir toutes NCF2 Kits ELISA
    NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human NCF2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product NCF2 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes NCF2 Kits ELISA
    NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))
    Autre désignation
    NCF2 (NCF2 Produits)
    Synonymes
    MGC81877 Kit ELISA, zgc:158405 Kit ELISA, noxa2 Kit ELISA, p67-phox Kit ELISA, p67phox Kit ELISA, NCF-2 Kit ELISA, NOXA2 Kit ELISA, P67-PHOX Kit ELISA, P67PHOX Kit ELISA, Ncf-2 Kit ELISA, neutrophil cytosolic factor 2 Kit ELISA, neutrophil cytosolic factor 2 L homeolog Kit ELISA, neutrophil cytosol factor 2 Kit ELISA, hypothetical protein Kit ELISA, NCF2 Kit ELISA, ncf2.L Kit ELISA, ncf2 Kit ELISA, p67phox Kit ELISA, VDBG_06643 Kit ELISA, PGTG_05816 Kit ELISA, LOC100136240 Kit ELISA, Ncf2 Kit ELISA
    ID gène
    4688
    UniProt
    P19878
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