Tetraspanin 7 Kit ELISA

N° du produit ABIN6951346

Détail du produit Tetraspanin 7 Kit ELISA

Antigène: Tetraspanin 7 (TSPAN7)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse TSPAN7 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: Tetraspanin 7 (TSPAN7)

Autre désignation: TSPAN7 (TSPAN7 Produits)

Synonymes: A15 Kit ELISA, CCG-B7 Kit ELISA, CD231 Kit ELISA, DXS1692E Kit ELISA, MRX58 Kit ELISA, MXS1 Kit ELISA, TALLA-1 Kit ELISA, TM4SF2 Kit ELISA, TM4SF2b Kit ELISA, 1200014P11Rik Kit ELISA, AI323365 Kit ELISA, Cd231 Kit ELISA, Mxs1 Kit ELISA, PE31 Kit ELISA, R74651 Kit ELISA, TALLA Kit ELISA, Tm4sf2 Kit ELISA, tm4sf2 Kit ELISA, zgc:63823 Kit ELISA, wu:fa11g01 Kit ELISA, wu:fd18e11 Kit ELISA, wu:fl39g07 Kit ELISA, a15 Kit ELISA, ccg-b7 Kit ELISA, cd231 Kit ELISA, mrx58 Kit ELISA, mxs1 Kit ELISA, talla-1 Kit ELISA, tspan7 Kit ELISA, tetraspanin 7 Kit ELISA, tetraspanin 7 L homeolog Kit ELISA, TSPAN7 Kit ELISA, Tspan7 Kit ELISA, tspan7 Kit ELISA, tspan7.L Kit ELISA

ID gène: 21912

UniProt: Q62283