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CRIP2 Kit ELISA

CRIP2 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951411
  • Antigène Tous les produits CRIP2
    CRIP2 (Cysteine-Rich Protein 2 (CRIP2))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human CRIP2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Tous les produits CRIP2
    CRIP2 (Cysteine-Rich Protein 2 (CRIP2))
    Autre désignation
    CRIP2 (CRIP2 Produits)
    Synonymes
    CRIP Kit ELISA, CRP2 Kit ELISA, ESP1 Kit ELISA, C6orf210 Kit ELISA, COQ10D3 Kit ELISA, DLP1 Kit ELISA, bA59I9.3 Kit ELISA, hDLP1 Kit ELISA, AW551867 Kit ELISA, Crp2 Kit ELISA, SmLim Kit ELISA, SmLIM Kit ELISA, zgc:77740 Kit ELISA, 0610010I23Rik Kit ELISA, AW743261 Kit ELISA, C77570 Kit ELISA, CRP4 Kit ELISA, Crp Kit ELISA, Hlp Kit ELISA, wu:fb30h10 Kit ELISA, zgc:55408 Kit ELISA, cysteine rich protein 2 Kit ELISA, decaprenyl diphosphate synthase subunit 2 Kit ELISA, cysteine and glycine-rich protein 2 Kit ELISA, cysteine and glycine rich protein 2 Kit ELISA, cysteine-rich protein 2 Kit ELISA, CRIP2 Kit ELISA, PDSS2 Kit ELISA, Csrp2 Kit ELISA, csrp2 Kit ELISA, CSRP2 Kit ELISA, Crip2 Kit ELISA, crip2 Kit ELISA
    ID gène
    1397
    UniProt
    P52943
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