PPIL2 Kit ELISA

N° du produit ABIN6951429

Détail du produit PPIL2 Kit ELISA

Antigène: PPIL2 (Peptidylprolyl Isomerase (Cyclophilin)-Like 2 (PPIL2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human PPIL2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: PPIL2 (Peptidylprolyl Isomerase (Cyclophilin)-Like 2 (PPIL2))

Autre désignation: PPIL2 (PPIL2 Produits)

Synonymes: cyc4 Kit ELISA, cyp60 Kit ELISA, hcyp-60 Kit ELISA, CYC4 Kit ELISA, CYP60 Kit ELISA, Cyp-60 Kit ELISA, UBOX7 Kit ELISA, hCyP-60 Kit ELISA, 0610009L05Rik Kit ELISA, 1700016N17Rik Kit ELISA, 4921520K19Rik Kit ELISA, 4930511F14Rik Kit ELISA, AA589416 Kit ELISA, C130078A06Rik Kit ELISA, si:dkeyp-86g2.1 Kit ELISA, zgc:56616 Kit ELISA, zgc:86735 Kit ELISA, peptidylprolyl isomerase like 2 S homeolog Kit ELISA, peptidylprolyl isomerase like 2 Kit ELISA, peptidylprolyl isomerase (cyclophilin)-like 2 Kit ELISA, ppil2.S Kit ELISA, PPIL2 Kit ELISA, ppil2 Kit ELISA, Ppil2 Kit ELISA

ID gène: 23759

UniProt: Q13356