NDRG1 Kit ELISA

N° du produit ABIN6951436

Détail du produit NDRG1 Kit ELISA

Antigène: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human NDRG1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Autre désignation: NDRG1 (NDRG1 Produits)

Synonymes: CAP43 Kit ELISA, CMT4D Kit ELISA, DRG-1 Kit ELISA, DRG1 Kit ELISA, GC4 Kit ELISA, HMSNL Kit ELISA, NDR1 Kit ELISA, NMSL Kit ELISA, PROXY1 Kit ELISA, RIT42 Kit ELISA, RTP Kit ELISA, TARG1 Kit ELISA, TDD5 Kit ELISA, Ndr1 Kit ELISA, Ndrl Kit ELISA, cap43 Kit ELISA, cmt4d Kit ELISA, drg1 Kit ELISA, gc4 Kit ELISA, hmsnl Kit ELISA, ndr1 Kit ELISA, ndrg1 Kit ELISA, nmsl Kit ELISA, proxy1 Kit ELISA, rit42 Kit ELISA, rtp Kit ELISA, targ1 Kit ELISA, tdd5 Kit ELISA, xndrg1 Kit ELISA, ndrg4 Kit ELISA, cb775 Kit ELISA, wu:fb60h02 Kit ELISA, zgc:63944 Kit ELISA, NDRG1 Kit ELISA, NON-RACE SPECIFIC DISEASE RESISTANCE PROTEIN Kit ELISA, non race-specific disease resistance 1 Kit ELISA, ndrg1-a Kit ELISA, ndrg1-b Kit ELISA, xNDRG1-A Kit ELISA, ndrg1l Kit ELISA, zgc:73047 Kit ELISA, N-myc downstream regulated 1 Kit ELISA, N-myc downstream regulated gene 1 Kit ELISA, N-myc downstream regulated 1 S homeolog Kit ELISA, N-myc downstream regulated 1a Kit ELISA, Late embryogenesis abundant (LEA) hydroxyproline-rich glycoprotein family Kit ELISA, N-myc downstream regulated 1 L homeolog Kit ELISA, N-myc downstream regulated 1b Kit ELISA, NDRG1 Kit ELISA, Ndrg1 Kit ELISA, ndrg1.S Kit ELISA, ndrg1 Kit ELISA, ndrg1a Kit ELISA, NDR1 Kit ELISA, ndrg1.L Kit ELISA, ndrg1b Kit ELISA

ID gène: 10397

UniProt: Q92597