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DHRS9 Kit ELISA

DHRS9 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951437
  • Antigène Tous les produits DHRS9
    DHRS9 (Dehydrogenase/reductase (SDR Family) Member 9 (DHRS9))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human DHRS9 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Tous les produits DHRS9
    DHRS9 (Dehydrogenase/reductase (SDR Family) Member 9 (DHRS9))
    Autre désignation
    DHRS9 (DHRS9 Produits)
    Synonymes
    3ALPHA-HSD Kit ELISA, RDH-TBE Kit ELISA, RDH15 Kit ELISA, RDHL Kit ELISA, RDHTBE Kit ELISA, RETSDR8 Kit ELISA, SDR9C4 Kit ELISA, Rdhl Kit ELISA, C730025I08Rik Kit ELISA, Rdh15 Kit ELISA, rdhl Kit ELISA, 3alpha-hsd Kit ELISA, rdh15 Kit ELISA, retsdr8 Kit ELISA, DHRS9 Kit ELISA, RDHA Kit ELISA, rdh1l Kit ELISA, wu:fb64b07 Kit ELISA, zgc:73286 Kit ELISA, dehydrogenase/reductase 9 Kit ELISA, dehydrogenase/reductase (SDR family) member 9 Kit ELISA, dehydrogenase/reductase (SDR family) member 9 L homeolog Kit ELISA, DHRS9 Kit ELISA, Dhrs9 Kit ELISA, dhrs9.L Kit ELISA, dhrs9 Kit ELISA
    ID gène
    10170
    UniProt
    Q9BPW9
    Pathways
    C21-Steroid Hormone Metabolic Process
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