Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) Kit ELISA
CMPK1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951480
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Antigène
Voir toutes Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) Kits ELISA
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Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1)
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Reactivité
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Humain
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Human CMPK1 ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Indications d'application
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Optimal working dilution should be determined by the investigator.
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Date de péremption
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6 months
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Antigène
Voir toutes Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) Kits ELISA
-
Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1)
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Autre désignation
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CMPK1 (CMPK1 Produits)
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Synonymes
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CK Kit ELISA, CMK Kit ELISA, CMPK Kit ELISA, UMK Kit ELISA, UMP-CMPK Kit ELISA, UMPK Kit ELISA, UMP/CMPK Kit ELISA, PSPTO1749 Kit ELISA, cmpk1 Kit ELISA, fb52d08 Kit ELISA, fk47a07 Kit ELISA, ump Kit ELISA, wu:fb52d08 Kit ELISA, wu:fk47a07 Kit ELISA, zgc:56452 Kit ELISA, Cmpk Kit ELISA, RGD1310116 Kit ELISA, cmpk Kit ELISA, 0610011D08Rik Kit ELISA, UMP-CMPk Kit ELISA, UCK Kit ELISA, cytidine/uridine monophosphate kinase 1 Kit ELISA, cytidylate kinase Kit ELISA, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic Kit ELISA, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic S homeolog Kit ELISA, cytidylate kinase Cmk Kit ELISA, cytidine monophosphate (UMP-CMP) kinase 1 Kit ELISA, CMPK1 Kit ELISA, cmk Kit ELISA, cmpk1 Kit ELISA, cmpk Kit ELISA, Cmpk1 Kit ELISA, cmpk1.S Kit ELISA, Mrub_0478 Kit ELISA, MMAH_RS06670 Kit ELISA, TAGG_RS04320 Kit ELISA, Trad_0784 Kit ELISA, Deba_2713 Kit ELISA, Toce_1154 Kit ELISA, VDIS_RS03585 Kit ELISA, Fbal_1939 Kit ELISA, Ilyop_1379 Kit ELISA, Lbys_3481 Kit ELISA, Calni_0093 Kit ELISA, Ftrac_1556 Kit ELISA, Ocepr_1777 Kit ELISA
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ID gène
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51727
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UniProt
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P30085
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Pathways
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Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
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