ATG16L1 Kit ELISA

N° du produit ABIN6951527

Détail du produit ATG16L1 Kit ELISA

Antigène: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ATG16L1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Autre désignation: ATG16L1 (ATG16L1 Produits)

Synonymes: APG16L Kit ELISA, ATG16A Kit ELISA, ATG16L Kit ELISA, IBD10 Kit ELISA, WDR30 Kit ELISA, 1500009K01Rik Kit ELISA, Apg16l Kit ELISA, Atg16l Kit ELISA, Wdr30 Kit ELISA, atg16 Kit ELISA, atg16l Kit ELISA, fb57e05 Kit ELISA, wu:fb57e05 Kit ELISA, zgc:110147 Kit ELISA, autophagy related 16 like 1 Kit ELISA, autophagy related 16-like 1 (S. cerevisiae) Kit ELISA, autophagy related 16-like 1 Kit ELISA, autophagy related 16 like 1 S homeolog Kit ELISA, ATG16 autophagy related 16-like 1 (S. cerevisiae) Kit ELISA, ATG16L1 Kit ELISA, Atg16l1 Kit ELISA, atg16l1 Kit ELISA, atg16l1.S Kit ELISA

ID gène: 55054

UniProt: Q676U5

Pathways: Autophagy