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CARHSP1 Kit ELISA

CARHSP1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951546
  • Antigène Voir toutes CARHSP1 Kits ELISA
    CARHSP1 (Calcium Regulated Heat Stable Protein 1, 24kDa (CARHSP1))
    Reactivité
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human CARHSP1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes CARHSP1 Kits ELISA
    CARHSP1 (Calcium Regulated Heat Stable Protein 1, 24kDa (CARHSP1))
    Autre désignation
    CARHSP1 (CARHSP1 Produits)
    Synonymes
    wu:fc04b03 Kit ELISA, zgc:65920 Kit ELISA, CARHSP1 Kit ELISA, csdc1 Kit ELISA, MGC53487 Kit ELISA, crhsp-24 Kit ELISA, 1200011K09Rik Kit ELISA, Crhsp-24 Kit ELISA, D16Ertd465e Kit ELISA, Crhsp24 Kit ELISA, CRHSP-24 Kit ELISA, CSDC1 Kit ELISA, calcium regulated heat stable protein 1 Kit ELISA, calcium regulated heat stable protein 1, 24kDa Kit ELISA, carhsp1 Kit ELISA, CARHSP1 Kit ELISA, Carhsp1 Kit ELISA
    ID gène
    23589
    UniProt
    Q9Y2V2
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