CES5A Kit ELISA

N° du produit ABIN6951576

Détail du produit CES5A Kit ELISA

Antigène: CES5A (Carboxylesterase 5A (CES5A))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse CES5A ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: CES5A (Carboxylesterase 5A (CES5A))

Autre désignation: CES5A (CES5A Produits)

Synonymes: CAUXIN Kit ELISA, CES4C1 Kit ELISA, CES5 Kit ELISA, CES7 Kit ELISA, 1700081L16Rik Kit ELISA, 1700122C07Rik Kit ELISA, BB081581 Kit ELISA, Ces7 Kit ELISA, Gm503 Kit ELISA, cauxin Kit ELISA, Ces5 Kit ELISA, LOC445455 Kit ELISA, carboxylesterase 5A Kit ELISA, CES5A Kit ELISA, Ces5a Kit ELISA

ID gène: 67935

UniProt: Q6AW46