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IL20RB Kit ELISA

IL20RB Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951643
  • Antigène Voir toutes IL20RB Kits ELISA
    IL20RB (Interleukin 20 Receptor beta (IL20RB))
    Reactivité
    • 2
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Rat IL-20 R beta ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product IL20RB Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes IL20RB Kits ELISA
    IL20RB (Interleukin 20 Receptor beta (IL20RB))
    Autre désignation
    IL-20 R beta (IL20RB Produits)
    Synonymes
    DIRS1 Kit ELISA, FNDC6 Kit ELISA, IL-20R2 Kit ELISA, AV228068 Kit ELISA, Fndc6 Kit ELISA, Gm186 Kit ELISA, Il20R2 Kit ELISA, IL20R2 Kit ELISA, RGD1566151 Kit ELISA, interleukin 20 receptor subunit beta Kit ELISA, interleukin 20 receptor beta Kit ELISA, IL20RB Kit ELISA, Il20rb Kit ELISA
    ID gène
    501043
    UniProt
    B8K1X9
    Pathways
    Regulation of Leukocyte Mediated Immunity
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