ATG12 Kit ELISA

N° du produit ABIN6951723

Détail du produit ATG12 Kit ELISA

Antigène: ATG12 (Autophagy Related 12 (ATG12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ATG12 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: ATG12 (Autophagy Related 12 (ATG12))

Autre désignation: ATG12 (ATG12 Produits)

Synonymes: APG12 Kit ELISA, APG12L Kit ELISA, FBR93 Kit ELISA, HAPG12 Kit ELISA, Apg12l Kit ELISA, AUTOPHAGY 12 Kit ELISA, AUTOPHAGY 12 B Kit ELISA, CG10861 Kit ELISA, Dmel\\CG10861 Kit ELISA, atg12 Kit ELISA, ATG12 Kit ELISA, apg12 Kit ELISA, DDBDRAFT_0185323 Kit ELISA, DDBDRAFT_0191412 Kit ELISA, DDB_0185323 Kit ELISA, DDB_0191412 Kit ELISA, 4931423H11Rik Kit ELISA, A330058M13Rik Kit ELISA, Atg12l Kit ELISA, AO090001000593 Kit ELISA, autophagy related 12 Kit ELISA, Ubiquitin-like superfamily protein Kit ELISA, Atg12p Kit ELISA, autophagy protein Kit ELISA, ubiquitin-like protein ATG12B Kit ELISA, autophagy associated protein Atg12 Kit ELISA, Autophagy-related 12 Kit ELISA, ATG12 autophagy related 12 homolog (S. cerevisiae) Kit ELISA, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene Kit ELISA, autophagy associated ubiquitin-like modifier Atg12 Kit ELISA, hypothetical protein Kit ELISA, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) Kit ELISA, autophagy protein 12 Kit ELISA, autophagy related 12 S homeolog Kit ELISA, autophagy-related protein 12 Kit ELISA, ATG12 Kit ELISA, Atg12 Kit ELISA, APG12B Kit ELISA, APG12 Kit ELISA, LOC9321066 Kit ELISA, apg12 Kit ELISA, atg12 Kit ELISA, LOC743425 Kit ELISA, CAALFM_C113950CA Kit ELISA, atg12.S Kit ELISA, ANI_1_922094 Kit ELISA, AOR_1_1064164 Kit ELISA

ID gène: 9140

UniProt: O94817

Pathways: Autophagy