ACE2 Kit ELISA

N° du produit ABIN6952471

Ce kit ELISA Humain ACE2 est un kit ELISA Colorimetric conçu pour quantifier Humain ACE2.

Aperçu rapide pour ACE2 Kit ELISA (ABIN6952471)

Antigène: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.025 ng/mL - 20 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Cells, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Détail du produit ACE2 Kit ELISA

Seuil minimal de détection: 0.025 ng/mL

Fonction: Human ACE-2 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects Human ACE-2. Other species not determined.

Attributs du produit: Human ACE-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water/li>
• Precision pipettes to deliver 2 μL to 1 μL volumes/li>
• Adjustable 1-25 μL pipettes for reagent preparation/li>
• 100 μL and 1 liter graduated cylinders/li>
• Tubes to prepare standard and sample dilutions/li>
• Absorbent paper/li>
• Microplate reader capable of measuring absorbance at 450nm/li>
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation de l'échantillon:

Recommended Dilution (serum & plasma):8 fold

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Détail du antigène

Antigène: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Autre désignation: ACE-2

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour