ACE2 Kit ELISA
Aperçu rapide pour ACE2 Kit ELISA (ABIN6952503)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- This assay employs a two-site sandwich ELISA to quantitate ACE2 in samples.
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Analytical Method
- Quantitative
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 200 μL
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Plaque
- Pre-coated
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Protocole
- An antibody specific for ACE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Restrictions
- For Research Use only
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- ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
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Autre désignation
- ACE2
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Pathways
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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