SARS-CoV-2 Nucleocapsid Kit ELISA

N° du produit ABIN6952756

Détail du produit SARS-CoV-2 Nucleocapsid Kit ELISA

Antigène: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Reactivité: Humain, SARS Coronavirus-2 (SARS-CoV-2)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.07 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.07 ng/mL

Application: ELISA

Fonction: The COVID-19 / SARS-COV-2 Nucleocapsid Protein ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of COVID-19 N Protein in serum (plasma is not recommended in this assay) and cell culture supernatants.

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects virus COVID-19 N Protein

Attributs du produit: COVID-19 N-Protein ELISA

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for COVID-19 N Protein coate on a 96-well plate. Standards and samples are pipetted into the wells and COVID-19 N Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-COVID-19 N Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID-19 N Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Procédure de l'essai:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Calcul des résultats:

Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 12 months

Détail du antigène

Antigène: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Autre désignation: SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Produits)

Synonymes: nucleocapsid phosphoprotein Kit ELISA, N Kit ELISA

Classe de substances: Viral Protein

Sujet: The spike protein (S-protein) and nucleocapsid protein (N-protein) are encoded by all coronaviruses, including the coronavirus (COVID-19). Nucleocapsid protein is a most abundant protein of coronavirus. The nucleocapsid protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N protein of coronavirus is chosen as a diagnostic tool since its strong immunogenicity.