SARS-CoV-2 N-Protein IgG Antibody Kit ELISA

N° du produit ABIN6952759

Le kit ELISA SARS-CoV-2 N-Protein IgG Antibody (ABIN6952759) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain SARS-CoV-2 N-Protein IgG Antibody.

Aperçu rapide pour SARS-CoV-2 N-Protein IgG Antibody Kit ELISA (ABIN6952759)

Antigène: SARS-CoV-2 N-Protein IgG Antibody

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Dried Blood, Plasma, Saliva, Serum

Détail du produit

Fonction: COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgG antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Analytical Method: Semi-Quantitative

Attributs du produit: COVID-19 N-Protein Human IgG ELISA Kit

Ingrédients:

• SARS-CoV-2 N-protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent
• 1X PBS (DBS version)
• Tween-20 (DBS version)
• Protease Inhibitor Cocktail (DBS version)

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Distilled or deionized water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Procédure de l'essai:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1.5 hours at room temperature.
3. Add 100 μL prepared HRP- anti positive control IgG Antibody and/or 100 μL of prepared HRP-anti Human IgG Antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
6. Read at 450 nm immediately.

Calcul des résultats:

Calibration curve: Calculate the mean absorbance for each set of duplicate Positive Control (Item C), samples, and subtract the average zero Positive Control optical density. Plot the calibration curve on log-log with Positive Control concentration (Unit/mL) on the xaxis and absorbance on the y-axis using Sigma plot or Excel software.

Restrictions: For Research Use only

Stockage

Stock: -80 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Date de péremption: 12 months

Détail du antigène

Antigène: SARS-CoV-2 N-Protein IgG Antibody

Classe de substances: Antibody

Sujet: Gene Symbols: IGG