Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

SARS-CoV-2 N-Protein IgM Antibody Kit ELISA

Reactivité: Humain, SARS Coronavirus-2 (SARS-CoV-2) Colorimetric Indirect ELISA Plasma, Serum
N° du produit ABIN6952760
  • Antigène
    SARS-CoV-2 N-Protein IgM Antibody
    Reactivité
    Humain, SARS Coronavirus-2 (SARS-CoV-2)
    Méthode de détection
    Colorimetric
    Type de méthode
    Indirect ELISA
    Application
    ELISA
    Fonction
    COVID-19 (SARS-CoV-2) N-Protein Human Antibody Detection Kit (Indirect ELISA method) for the semi-quantitative measurement of human IgM antibody against SARS-CoV-2 in serum or plasma.
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Semi-Quantitative
    Attributs du produit
    COVID-19 N-Protein Human IgM ELISA Kit
    Ingrédients
    • SARS-CoV-2 N-protein coated 96-well microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent
    • Positive Control
    • HRP-anti positive control IgG Antibody
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plaque
    Pre-coated
    Protocole
    This COVID19 human IgM antibody ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARSCoV-2 N protein, which combines with the corresponding antibody present in a sample. When a secondary anti-human Antibody-HRP is added, a complex of Antibody-HRPhuman IgM antibody-virus N antigen forms on the microplate. A TMB substrate is added and a blue color is generated. The depth of color is relative to the amount of the anti-SARS-CoV-2 IgM antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
    Procédure de l'essai
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL positive control or sample to each well. Incubate 1.5 hours at room temperature.
    3. Add 100 μL prepared HRP- anti positive control IgG Antibody and/or 100 μL of prepared HRP-anti Human IgG Antibody to each well. Incubate 1 hour at room temperature.
    4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
    5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
    6. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    12 months
  • Antigène
    SARS-CoV-2 N-Protein IgM Antibody
    Classe de substances
    Antibody
Vous êtes ici:
Support technique