Cytochrome P450 Kit ELISA

N° du produit ABIN6975465

Détail du produit Cytochrome P450 Kit ELISA

Antigène: Cytochrome P450 (CYP)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Fonction: For the quantitative determination of human cytochrome P450 concentrations in serum, plasma, tissue homogenates, cell lysates.

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of human cytochrome P450. No significant cross-reactivity or interference between human cytochrome P450 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human cytochrome P450 and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 7.8 pg/mL

Ingrédients:

• Assay plate
• Standard
• HRP-avidin (100 x concentrate)
• Biotin-antibody (100 x concentrate)
• Sample Diluent
• HRP-avidin Diluent
• Biotin-antibody Diluent
• Wash Buffer (25 x concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole:

1. Prepare reagents, samples and standards as instructed.
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
3. Remove the liquid of each well, don't wash.
4. Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
5. Aspirate and wash 3 times.
6. Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
7. Aspirate and wash 5 times.
8. Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
9. Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.

Préparation des réactifs:

1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
3. Wash Buffer(1x)- If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 2000 pg/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (2000 pg/mL). Sample Diluent serves as the zero standard (0 pg/mL).

Note:

• Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
• Bring all reagents to room temperature (18-25 °C) before use for 30 min.
• Prepare fresh standard for each assay. Use within 4 hours and discard after use.
• Making serial dilution in the wells directly is not permitted.
• Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
• Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Précision du teste: Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.

Date de péremption: 6 months

Détail du antigène

Antigène: Cytochrome P450 (CYP)

Autre désignation: Cytochrome P450 (CYP Produits)

Synonymes: CPF3 Kit ELISA, CYP4F Kit ELISA, LTB4H Kit ELISA, 6t1 Kit ELISA, CG1644 Kit ELISA, DmelCG1644 Kit ELISA, CYTOCHROME P450 Kit ELISA, FAMILY 705 Kit ELISA, POLYPEPTIDE 9 Kit ELISA, SUBFAMILY A Kit ELISA, T20P8.6 Kit ELISA, T20P8_6 Kit ELISA, cytochrome P450 Kit ELISA, family 71 Kit ELISA, polypeptide 13 Kit ELISA, subfamily A Kit ELISA, T11J7.16 Kit ELISA, T11J7_16 Kit ELISA, F13P17.22 Kit ELISA, family 710 Kit ELISA, polypeptide 2 Kit ELISA, CYP77A7 Kit ELISA, FAMILY 77 Kit ELISA, POLYPEPTIDE 7 Kit ELISA, UNE9 Kit ELISA, UNFERTILIZED EMBRYO SAC 9 Kit ELISA, POLYPEPTIDE 15 Kit ELISA, POLYPEPTIDE 18 Kit ELISA, T9E8.50 Kit ELISA, T9E8_50 Kit ELISA, polypeptide 20 Kit ELISA, DL3710C Kit ELISA, FCAALL.270 Kit ELISA, POLYPEPTIDE 1 Kit ELISA, CABBAGE 3 Kit ELISA, CBB3 Kit ELISA, CONSTITUTIVE PHOTOMORPHOGENIC DWARF Kit ELISA, CYP90 Kit ELISA, CYP90A Kit ELISA, CYP90A1 Kit ELISA, CYTOCHROME P450 90A1 Kit ELISA, CYTOCHROME P450 MONOOXYGENASE Kit ELISA, DWARF 3 Kit ELISA, DWF3 Kit ELISA, MJJ3.9 Kit ELISA, MJJ3_9 Kit ELISA, F25C20.24 Kit ELISA, F25C20_24 Kit ELISA, polypeptide 18 Kit ELISA, F22L4.14 Kit ELISA, F22L4_14 Kit ELISA, FAMILY 86 Kit ELISA, POLYPEPTIDE 4 Kit ELISA, F13D4.120 Kit ELISA, F13D4_120 Kit ELISA, family 82 Kit ELISA, polypeptide 1 Kit ELISA, subfamily F Kit ELISA, family 711 Kit ELISA, MAX1 Kit ELISA, MORE AXILLARY BRANCHES 1 Kit ELISA, T1D16.19 Kit ELISA, T1D16_19 Kit ELISA, F15K20.21 Kit ELISA, F15K20_21 Kit ELISA, family 94 Kit ELISA, subfamily C Kit ELISA, F13P17.35 Kit ELISA, family 98 Kit ELISA, polypeptide 3 Kit ELISA, T20B5.9 Kit ELISA, T20B5_9 Kit ELISA, family 712 Kit ELISA, T24P15.16 Kit ELISA, T24P15_16 Kit ELISA, family 704 Kit ELISA, T13E15.10 Kit ELISA, F17K2.4 Kit ELISA, F4I18.5 Kit ELISA, family 86 Kit ELISA, LACERATA Kit ELISA, LCR Kit ELISA, polypeptide 8 Kit ELISA, F14M4.22 Kit ELISA, family 709 Kit ELISA, subfamily B Kit ELISA, F14M4.21 Kit ELISA, family 87 Kit ELISA, polypeptide 9 Kit ELISA, T21P5.11 Kit ELISA, T21P5_11 Kit ELISA, F1C9.32 Kit ELISA, F1C9_32 Kit ELISA, POLYPEPTIDE 22 Kit ELISA, family 705 Kit ELISA, polypeptide 23 Kit ELISA, polypeptide 30 Kit ELISA, subfamily G Kit ELISA, family 76 Kit ELISA, subfamily D Kit ELISA, family 706 Kit ELISA, polypeptide 4 Kit ELISA, T4C9.140 Kit ELISA, T4C9_140 Kit ELISA, F18A5.160 Kit ELISA, F18A5_160 Kit ELISA, family 83 Kit ELISA, REDUCED EPIDERMAL FLUORESCENCE 2 Kit ELISA, REF2 Kit ELISA, DL3695C Kit ELISA, family 702 Kit ELISA, FCAALL.257 Kit ELISA, DL3700W Kit ELISA, FCAALL.266 Kit ELISA, DL3720W Kit ELISA, FCAALL.274 Kit ELISA, DL3725W Kit ELISA, FCAALL.277 Kit ELISA, 21-OH Kit ELISA, 21OH Kit ELISA, 21OHA Kit ELISA, 21OHB Kit ELISA, CYP21OH-A Kit ELISA, Cyp21 Kit ELISA, Cyp21-ps1 Kit ELISA, Cyp21B Kit ELISA, Cyp21a2-ps Kit ELISA, Cyp21a2ps Kit ELISA, Oh21-1 Kit ELISA, Oh21-2 Kit ELISA, CYPIID25 Kit ELISA, Cypiiia15 Kit ELISA, cytochrome P450 family 4 subfamily F member 3 Kit ELISA, CG1644 gene product from transcript CG1644-RA Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 9 Kit ELISA, cytochrome P450 family 71 polypeptide Kit ELISA, cytochrome P450, family 710, subfamily A, polypeptide 2 Kit ELISA, Cytochrome P450 superfamily protein Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 15 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 18 Kit ELISA, cytochrome P450, family 71, subfamily A, polypeptide 20 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 71, subfamily A, polypeptide 18 Kit ELISA, cytochrome P450, family 86, subfamily A, polypeptide 4 Kit ELISA, cytochrome P450, family 82, subfamily F, polypeptide 1 Kit ELISA, cytochrome P450, family 711, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 94, subfamily C, polypeptide 1 Kit ELISA, cytochrome P450, family 710, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 98, subfamily A, polypeptide 3 Kit ELISA, cytochrome P450, family 712, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 704, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 704, subfamily A, polypeptide 2 Kit ELISA, cytochrome P450, family 86, subfamily A, polypeptide 8 Kit ELISA, cytochrome P450, family 709, subfamily B, polypeptide 2 Kit ELISA, cytochrome P450, family 709, subfamily B, polypeptide 1 Kit ELISA, cytochrome P450, family 87, subfamily A, polypeptide 9 Kit ELISA, cytochrome P450, family 94, subfamily B, polypeptide 2 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 22 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 23 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 30 Kit ELISA, cytochrome P450, family 82, subfamily G, polypeptide 1 Kit ELISA, cytochrome P450, family 86, subfamily C, polypeptide 2 Kit ELISA, cytochrome P450, family 94, subfamily B, polypeptide 3 Kit ELISA, cytochrome P450, family 76, subfamily G, polypeptide 1 Kit ELISA, cytochrome P450, family 94, subfamily D, polypeptide 2 Kit ELISA, cytochrome P450, family 706, subfamily A, polypeptide 4 Kit ELISA, cytochrome P450, family 83, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 702, subfamily A, polypeptide 2 Kit ELISA, cytochrome P450, family 702, subfamily A, polypeptide 3 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 2 Kit ELISA, cytochrome P450, family 705, subfamily A, polypeptide 3 Kit ELISA, cytochrome P450, family 21, subfamily a, polypeptide 1 Kit ELISA, cytochrome P450, family 2, subfamily g, polypeptide 1 Kit ELISA, vitamin D3 25-Hydroxylase Kit ELISA, cytochrome P-450 Kit ELISA, cytochrome P450 Kit ELISA, olfactory receptor 1440-like Kit ELISA, CYP4F3 Kit ELISA, Cyp6t1 Kit ELISA, CYP705A9 Kit ELISA, CYP71A13 Kit ELISA, CYP710A2 Kit ELISA, UNE9 Kit ELISA, CYP705A15 Kit ELISA, CYP705A18 Kit ELISA, CYP71A20 Kit ELISA, CYP705A1 Kit ELISA, CPD Kit ELISA, CYP71A18 Kit ELISA, CYP86A4 Kit ELISA, CYP82F1 Kit ELISA, CYP711A1 Kit ELISA, CYP94C1 Kit ELISA, 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Sujet: CYP450

UniProt: P04798

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process