HCP Kit ELISA

N° du produit ABIN7538040

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain HCP.

Aperçu rapide pour HCP Kit ELISA (ABIN7538040)

Antigène: HCP (Host Cell Proteins (HCP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.125 ng/mL - 100 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant

Détail du produit HCP Kit ELISA

Seuil minimal de détection: 3.125 ng/mL

Fonction: The kit is intended for the quantitative determination of residual host cell protein in Cell Culture Supernatants, Protein Purification Process, and End-Product expressed by HEK 293 expression systems.

Analytical Method: Quantitative

Réactivité croisée (Details): No cross-reaction exists between the homologous antigens.

Sensibilité: 0.78 ng/mL

Attributs du produit: This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.

Ingrédients: Pre-Coated Microplate (Detachable), Standard (Stock Solution), Detection antibody (100x), TMB Substrates, Stop Solution, Wash Solution (100x), Diluent Buffer (10x), Plate Sealer

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 µL

Durée du test: 3 h

Plaque: Pre-coated

Précision du teste: Intra Variation%: 7.1-10.6, Inter Variation%: 6.8-10.4

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: HCP (Host Cell Proteins (HCP))

Autre désignation: Host Cell Proteins