VZV IgM Kit ELISA

N° du produit ABIN7666553

Kit ELISA VZV IgM Humain, Colorimetric test pour la quantification de Humain VZV IgM.

Aperçu rapide pour VZV IgM Kit ELISA (ABIN7666553)

Antigène: VZV IgM (Anti-Varicella Zoster Virus IgM (VZV IgM))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit VZV IgM Kit ELISA

Fonction: anti-VZV IgM - ELISA kit for detection IgM antibodies to varicella zoster virus (VZV) in human serum (plasma).

Marque: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Ingrédients:

• ELISA break-away strips coated with specific antigen STRIPS Ag 1 microplate
• 1.3 mL Calibrator, r.t.u. 1) CAL 1 vial
• 1.3 mL Negative control serum, r.t.u. NC 1 vial
• 1.3 mL Positive control serum, r.t.u. PC 1 vial
• 2 mL RF sorbent2), 25x concentrated RF SORB 25x 1 vial
• 13 mL Anti-human IgM antibodies labelled with horseradish peroxidase r.t.u. (Px-conjugate) CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate (TMB substrate), r.t.u. TMB 1 vial
• 13 mL Stop solution, r.t.u. STOP 1 vial
• Instruction manual
• Quality control certificate 1) ready to use 2) Goat anti-human IgG globulin

Matériel non inclus:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: anti-VZV IgM assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a native antigen. Anti-VZV antibodies from serum samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away, those that formed complexes with the antigens are later on recognised by animal anti-human IgM antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: VZV IgM (Anti-Varicella Zoster Virus IgM (VZV IgM))

Autre désignation: VZV IgM

Classe de substances: Antibody