CMV IgM Kit ELISA

N° du produit ABIN7666564

Le kit ELISA CMV IgM (ABIN7666564) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain CMV IgM.

Aperçu rapide pour CMV IgM Kit ELISA (ABIN7666564)

Antigène: CMV IgM (Anti-Cytomegalovirus IgM Antibody (CMV IgM))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit CMV IgM Kit ELISA

Fonction: anti-CMV IgM - ELISA kit for the detection of IgM antibodies to human cytomegalovirus (CMV) in serum.

Marque: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Ingrédients:

• ELISA 8-well break-away strips coated with a mixture of specific recombinant antigens STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control serum, r.t.u. PC 1 vial
• 2.0 mL Calibrator ,r.t.u. CAL 1 vial
• 13 mL Anti-human IgM antibodies labelled with horseraddish peroxidase r.t.u. CONJ-V 1 vial
• 55 mL Wash buffer 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
• 13 mL Stop solution r.t.u. STOP 1 vial
• 2 mL RF sorbent 2), 25x concentrated RF SORB 25x 1 vial
• Instruction manual
• Quality control certificate 1) (ready to use) 2) Goat anti-human IgG globulin /

Matériel non inclus:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: anti-CMV IgM assay is a solid-phase immunoanalytical test. The strips are coated with the mixture of recombinant antigens. Anti-CMV antibodies in serum samples bind to the mixture of immobilized antigens. Bound antibodies are later on recognised by animal anti-human IgM antibodies labelled with horseradish peroxidase. The amount of bound labeled antibodies is determined by color enzymatic reaction. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: The ELISA kit should be used within three months after opening. a. Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Under these conditions, the expiration period of the entire kit is indicated on the central label, the expiration date of the individual components is indicated on their package labels. b. Store unused strips in the sealable pouch and keep the desiccant inside. c. Kits are shipped in cooling bags, the transport time up to 72 hours have no influence on expiration. If you find damage at any part of the kit, please inform the manufacturer immediately. d. Store undiluted sera in aliquots at -18 to -28 °C. Frequent freezing and thawing is not recommended. Undiluted serum samples could be stored at +2 to +10°C up to one week. e. Do not store diluted samples. Always prepare fresh. 8

Détail du antigène

Antigène: CMV IgM (Anti-Cytomegalovirus IgM Antibody (CMV IgM))

Autre désignation: CMV IgM

Classe de substances: Antibody