C6 Kit ELISA

N° du produit ABIN7666567

Ce kit ELISA Humain C6 est un kit ELISA Colorimetric conçu pour quantifier Humain C6.

Aperçu rapide pour C6 Kit ELISA (ABIN7666567)

Antigène: C6 (Complement Component 6 (C6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit C6 Kit ELISA

Fonction: anti-C6 - ELISA kit for detection of antibodies against C6 protein (part of VlsE) in human serum/plasma.

Marque: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Ingrédients:

• ELISA break-away strips coated with specific recombinant antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u.1) NC 1 vial
• 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 13 mL Anti-human animal IgM+IgG antibodies labelled with horseradish peroxidase, r.t.u (anti IgM/IgG Px-conjugate) CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate (TMB substrate), r.t.u. (TMB/H2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• .Instruction manual
• Quality control certificate 1) r.t.u. ready to use

Matériel non inclus:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: anti-C6 is a solid-phase immunoanalytical test. The polystyrene strips are coated with the specific antigens. The anti-C6 antibodies, if present in the tested sera, bind to the immobilized antigen. The bonded antibodies react later on with antibodies against human IgM and IgG labelled with horseradish peroxidase. The presence of labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: C6 (Complement Component 6 (C6))

Autre désignation: C6

Classe de substances: Antibody