DBH Kit ELISA

N° du produit ABIN7671298

Le kit ELISA DBH (ABIN7671298) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat DBH.

Aperçu rapide pour DBH Kit ELISA (ABIN7671298)

Antigène: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Application: ELISA

Type d'échantillon: Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Classe de qualité: Quick

Détail du produit DBH Kit ELISA

Seuil minimal de détection: 0.781 ng/mL

Fonction: Rat Dbh (Dopamine beta-hydroxylase) QuickTest ELISA Kit

Analytical Method: Quantitative

Specificité: Specifically binds with Dbh , no obvious cross reaction with other analogues.

Sensibilité: 0.469 ng/mL

Ingrédients:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Cap/Det Ab (Ready to use
• blue)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Matériel non inclus:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Sandwich ELISA, Double Antibody

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: Step 1: Take out the required plate wells, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37 °C. Washing: Wash the plate twice without immersion. Step 2: Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37 °C. Washing: Wash the plate five times without immersion. Step 3: Add 90 µL TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Procédure de l'essai:

1. Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
2. Wash the plate twice without immersion.
3. Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
4. Wash the plate five times without immersion.
5. Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
6. Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid direct light exposure to antibody solutions

Stock: 4 °C

Stockage commentaire: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Date de péremption: 12 months

Détail du antigène

Antigène: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Autre désignation: Dbh

Sujet: Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, Soluble dopamine beta-hydroxylase, DBH

UniProt: Q05754

Pathways: Carbohydrate Homeostasis