CCR5 Kit ELISA
Aperçu rapide pour CCR5 Kit ELISA (ABIN7830480)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- CCR5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CCR5 expression in cultured cells.
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Analytical Method
- Qualitative
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Uncoated
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Protocole
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Store the kit at 4°C.
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- CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))
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Autre désignation
- CCR5
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Sujet
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Synonyms: CC-CKR-5|CCCKR5|CD195|CKR-5|CKR5|CMKBR5|IDDM22
Gene Description: chemokine (C-C motif) receptor 5
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ID gène
- 1234
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UniProt
- P51681
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Pathways
- Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size
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