IL-10RA Kit ELISA
Aperçu rapide pour IL-10RA Kit ELISA (ABIN7830601)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- IL10RA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IL10RA expression in cultured cells.
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Analytical Method
- Qualitative
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Uncoated
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Protocole
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Store the kit at 4°C.
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- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
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Autre désignation
- IL10RA
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Sujet
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Synonyms: CDW210A|HIL-10R|IL-10R1|IL10R
Gene Description: interleukin 10 receptor, alpha
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ID gène
- 3587
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UniProt
- Q13651
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Pathways
- Growth Factor Binding
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